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primary normal human lung fibroblast nhlf  (Lonza)


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    Lonza primary normal human lung fibroblast nhlf
    Primary Normal Human Lung Fibroblast Nhlf, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+lung+fibroblasts+(nhlf)/normal+human+lung+fibroblasts++nhlfs+/pm40595437-254-0-9
    Average 90 stars, based on 1 article reviews
    primary normal human lung fibroblast nhlf - by Bioz Stars, 2026-11
    90/100 stars

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    Cell Culture:

    Article Title: Viscosity regulates cell spreading and cell-extracellular matrix interactions.
    Article Snippet: doi:10.1111/febs.17306 Fluid viscosity and osmolarity are among some of the underappreciated mechanical stimuli that cells can detect.. Abnormal changes of multiple fluidic factors such as viscosity and osmolarity have been linked with diseases such as cystic fibrosis, cancer, and coronary heart disease.. Changes in viscosity have been recently suggested as a regulator of cell locomotion.

    Article Title: Development of a novel single channel arteriole microphysiological system for characterizing leukocyte-endothelial interactions
    Article Snippet: EC were cultured on gelatin coated cell culture flasks in EGM-2 (Lonza, CC3162) medium. .. Normal human lung fibroblasts (NHLF, Lonza) were cultured in DMEM (Corning, 10027CV) with 5% FBS (VWR, S1700-100). .. Both HUVEC and NHLF were transduced with lentiviruses encoding fluorescent proteins (mCherry/Addgene, 36084 or Azurite/Addgene, 36086) and were used between passages 5-9.

    Article Title: Low levels of physiological interstitial flow eliminate morphogen gradients and guide angiogenesis
    Article Snippet: .. Cell culture Normal human lung fibroblasts (NHLF) were obtained from Lonza (Allendale, NJ), and were cultured up to seven passages in complete fibroblast growth medium (FGM; Lonza). ..

    Concentration Assay:

    Article Title: A defined clathrin-mediated trafficking pathway regulates sFLT1/VEGFR1 secretion from endothelial cells.
    Article Snippet: Briefly, control HUVEC or HUVEC 48 h post-siRNA treatment were incubated with cytodex 3 microcarrier beads (#17048501, GE Healthcare Life Sciences) overnight then resuspended in 2.2 mg/ mL fibrinogen (#820224, Fisher) plus aprotinin (A3428, Sigma) and embedded in a fibrin matrix by combining 7 μl of 50U/ml thrombin (T7201-500UN, Sigma) with 500 μl of bead/fibrinogen solution in a 24-well glass-bottomed plate (#662892, Grenier Bio) that was incubated for 30 min at RT then 30 min at 37 °C. .. EGM-2 and normal human lung fibroblasts (NHLF, CC2512, Lonza) at a concentration of 2 × 105 cells/mL was added to each well, and incubation was at 37 °C. ..

    Incubation:

    Article Title: A defined clathrin-mediated trafficking pathway regulates sFLT1/VEGFR1 secretion from endothelial cells.
    Article Snippet: Briefly, control HUVEC or HUVEC 48 h post-siRNA treatment were incubated with cytodex 3 microcarrier beads (#17048501, GE Healthcare Life Sciences) overnight then resuspended in 2.2 mg/ mL fibrinogen (#820224, Fisher) plus aprotinin (A3428, Sigma) and embedded in a fibrin matrix by combining 7 μl of 50U/ml thrombin (T7201-500UN, Sigma) with 500 μl of bead/fibrinogen solution in a 24-well glass-bottomed plate (#662892, Grenier Bio) that was incubated for 30 min at RT then 30 min at 37 °C. .. EGM-2 and normal human lung fibroblasts (NHLF, CC2512, Lonza) at a concentration of 2 × 105 cells/mL was added to each well, and incubation was at 37 °C. ..

    other:

    Article Title: The TRPA1 cation channel is upregulated by cigarette smoke in mouse and human macrophages modulating lung inflammation
    Article Snippet: Differentiation of macrophages was induced using 10 nM Phorbol 12-myristate 13-acetate (Sigma-Aldrich, Saint Louis, USA) at 37 °C with 5% CO 2 for 5 h.

    Transduction:

    Article Title: Dynamic reporters for probing real-time activation of human fibroblasts from single cells to populations
    Article Snippet: .. Normal human lung fibroblasts (NHLF, Lonza) were transduced with the Stable reporter lentivirus at multiplicities of infection (MOI) of 1, 3, and 6, based on functional titers calculated using the model cell line NIH3T3. ..

    Infection:

    Article Title: Dynamic reporters for probing real-time activation of human fibroblasts from single cells to populations
    Article Snippet: .. Normal human lung fibroblasts (NHLF, Lonza) were transduced with the Stable reporter lentivirus at multiplicities of infection (MOI) of 1, 3, and 6, based on functional titers calculated using the model cell line NIH3T3. ..

    Functional Assay:

    Article Title: Dynamic reporters for probing real-time activation of human fibroblasts from single cells to populations
    Article Snippet: .. Normal human lung fibroblasts (NHLF, Lonza) were transduced with the Stable reporter lentivirus at multiplicities of infection (MOI) of 1, 3, and 6, based on functional titers calculated using the model cell line NIH3T3. ..



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